Bacteria as Potential Indicators of Heavy Metal Contamination in a Tropical Mangrove and the Implications on Environmental and Human Health

Melanie De La Rosa- Acosta, Johannys Jiménez-Collazo, Marixa Maldonado-Román, Karlo Malavé-Llamas, Juan Carlos Musa-Wasil

Abstract


Heavy metal (HM) exposure has been associated with human health diseases like cancer, kidney and liver damage, neurological disorders, motor skills, low bone density and learning problems. With the beginning of the industrialization the heavy metals in high concentration contributes to put on risk the humans in the vicinity. Our study site is located in Cataño, Puerto Rico, a highly industrialization area that has a recreational park nearby, a rum distillery, two thermoelectric factories, and was impacted by CAPECO (oil refinery) explosion in 2009. Las Cucharillas marsh is part of The San Juan Bay Estuary System, considered as a critical wildlife area because of their location. This mangrove marsh has three of the four mangrove species found in PR Laguncularia racemosa, Avicennia germinans and Rhizophora mangle; species that have the capacity to phytoremediate HM. This study was aimed at seven different heavy metals: Arsenic (As), Cadmium (Cd), Chromium (Cr), Lead (Pb), Zinc (Zn), Mercury (Hg) and Copper (Cu). These metals at high concentrations are of human health concern due to their toxicity, persistence, bioaccumulative and biomagnification potentials. Contamination of surface sediments with HM affects the food chain, starting with marine organisms up to humans. The people who live near the contaminated area and the local fishermen are at high risk of exposure. Studies reveal that certain microorganisms can resist the toxicity of heavy metals even at high concentrations. Our study pretends to exploit the sensitive nature of some bacteria to HM and use them as bioindicators. The objective of this research is to assess the bacterial community on the mangrove marsh, identify these bacteria and correlate bacterial species with the type and concentration of the metals found on the site. Our preliminary results with the BIOLOG® identification were five bacteria that are: Carnobacterium inhibens, Cupriavidus gilardii, Enterococcus maloduratus, Microbacterium flavescens and Ralstonia pickettii. This study will continue with an assessment of the exposure of different concentrations of heavy metals to our identified bacteria and underlying the mechanisms of degradation, magnification and or bioconcentration of these heavy metals.

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REFERENCES

González Mendoza D, Juárez Grimaldo O, Cervantes Díaz L. Los Elementos Potencialmente Tóxicos en las Plantas de Manglar: Una Revisión de los Mecanismos de Tolerancia Involucrados. Revista Interciencia 2008; INCI 33 (11).

World Health Organization Ten Chemicals of Major Concern (2013). [http://www.who.int/ipcs/assessment/public_health/chemicals_phc/en/]

Krzyzanowsky J. Environmental Pathways of Potential Impact to Human Health from Oil and Gas Development in Northeast British, Canada. Environ: Rev 2012; 20:122-134.

Registro Central de Cáncer de Puerto Rico: Incidencia de Cáncer en Puerto Rico [http://www.rcpr.org/]

Kamika I, Momba NB M. Assessing the Resistance and Bioremediation Ability of Selected Bacterial and Protozoan Species to Heavy Metals in Metal-Rich Industrial Waste Water. BMC Microbiology 2013; 13:1471-2180.

Luo W, Lu Y, Wan T, Hu W, Jiao W, Naile J, Khim J, Giesy JP. Ecological Risk Assessment of Arsenic and Metals in Sediments of Coastal Areas of Northern Bohai and Yellow Seas, China. AMBIO 2010; 39 5-6: 367-375.

Zakaria ZA, Jaapar J, Ahmad WA. Bacteria as Bioindicators for Metal Contamination. Biomonitoring in Tropical Coastal Ecosystems. Phang & Brown 2004; 131-135.

Rathnayake VN, Megharaj M, Bolan N, Naidu R. Tolerance of Heavy Metals by Gram Positive Soil Bacteria. World Academy of Science, Engineering Technology 2009; 53: 1185-1189.

Morales Agrinzoni C. Perspectiva Histórica de la Ciénaga Las Cucharillas. Perspectivas en Asuntos Ambientales 2013; 2 (1):1-162.

Mejías CL, Musa JC, Otero J. Exploratory Evaluation of Retranslocation and Bioconcentration of Heavy Metals in Three Species of Mangrove at Las Cucharillas Marsh, Puerto Rico. The Journal of Tropical Life Sciences 2013; 3 (1): 14-22

Historia de la Casa Bacardi [www.casabacardi.org]

Vullo D. Microorganismos y Metales Pesados: Una Interacción en Beneficio del Medio Ambiente. Revista Química Viva 2003; 3 (2): 93-104.

Sotomayor-Rivera C, Zayas B, González E, Musa-Wasil JC. Evaluación de la Presencia de Metales Pesados en la Ciénaga Las Cucharillas. Perspectivas en Asuntos Ambientales 2013; 2 (1): 1-162.

Environmental Protection Agency EPA sediment sampling methods SOP #EH-02. [http://www2.epa.gov/sites/production/files/documents/r8-src_eh-02.pdf].

Environmental Protection Agency EPA, 1999, Field Sampling

Guidance Document #1215 Sediment Sampling, Rev. 1, US EPA Region 9

Laboratory. [http://www.epa.gov/region6/qa/qadevtools/mod5_sops/sediment_sampling/r9-sedimentsample_gui.pdf].

Environmental Protection Agency EPA, 1999, Field Sampling Guidance Document #1230 Sampling Equipment Decontamination, Rev. 1, US EPA Region 9 Laboratory. [http://www.epa.gov/region6/qa/qadevtools/mod5_sops/misc_field_procudures/deconhazwaste_gui.pdf].

Schumacher BA, Shines KC, Burton JV, Papp ML. A Comparison of Soil Sample Homogenization Techniques. Environmental Protection Agency 1990; EPA (600).

McFeters G, Stuart D. Survival of Coliform Bacteria in Natural Waters: Field and Laboratory Studies with Membrane-Filter Chambers. Applied Microbiology 1972; 24 (5): 805-811.

Suginaka H, Ichikawa A, Kotani S. Penicillin-Resistant Mechanisms in Pseudomonas aeruginosa. Binding of Penicillin to Pseudomonas aeruginosa KM 338. Antimicrobial Agents and Chemotherapy 1975; 7 (5): 629-635.

Córdova-Kreylos AL, Cao Y, Green PG, Hwang HM, Kvivila KM, La Montagne MG, Van De Werfhrst LC, Holden PA, Scow KM. Diversity, Composition and Geographical Distribution of Microbial Communities in California Salt Marsh Sediments. Applied and Environmental Microbiology 2006; 72 (5): 3357-3366.

Xie X, Fu J, Wang H, Liu J. Heavy Metal Resistance by Two Bacterial Strains Isolated from a Copper Mine Tailing in China. African Journal of Biotechnology 2010; 26: 4056-4066.

Abdelatey L, Khalil W, Ali T, Mahrous K. Heavy Metal Resistance and Gene Expression Analysis of Metal Resistance Genes in Gram Positive and Gram Negative Bacteria Present in Egyptian Soils. Journal of Applied Sciences in Environmental Sanitation 2011; 6(2): 201-211.

Fisher K, Phillips C. The Ecology, Epidemiology and Virulence of Enterococcus. Microbiology 2009; 155(6): 1749-1757.

Morrinson D, Woodford N, Cookson B. Enterococci as Emerging Pathogens of Humans. Journal of Applied Microbiology Symposium Supplement 1997; 83(1): 89-99.

Environmental Protection Agency EPA Fecal Bacteria. Water: Monitoring and Assessment [http://water.epa.gov/type/rsl/monitoring/vms511.cfm]

Byappanahalli M, Neevers M, KorajKic A, Staley Z. Harwood Enterococci in the Environment. Microbial and Molecular Biology Reviews 2012; 76(4): 685-706.

Arias C, Murray B. Mechanisms of Antibiotic Resistance in Enterococci (2013). [www.uptodate.com/contents/mechanisms-of-antibiotics-resistance-in-enterococci#H18]

Sood S, Malhotra M, Das BK, Kapil A. Enterococccal Infections and Antimicrobial Resistance. Indian Journal of Medicine Sciences 2008; 128: 111-121.

Tortoli E, Rindi L, Bartoloni A, Garzelli C, Manfrin V, Mantella A, Piccoli P, Scarparo C. Isolation of a Novel Sequevar of Mycobacterium flavescens from the Synovial Fluid of an AIDS Patient. Clinical Microbiology and Infection 2004; 10 (11): 1017-1019.

Leisner J, Laursen BG, Prevost H, Drider D, Dalgaar P. Carnobacterium: Positive and Negative Effects in the Environment and in foods. Federation of European Microbiological Societies 2007; (31): 592-613.

Coombs J, Brenchley JE. Characterization of Two New Glycosyl Hydrolases from the Lactic Acid Bacterium Carnobacterium piscícola Strain BA. Applied Environmental Microbiology 2001; 67: 5094-5099.

Simpson J, Santo Domingo JW, Reasoner D.J. Assessment of Equine Fecal Contamination: The Search for Alternative Bacterial Source-Tracking Targets. Federation of European Microbiological Societies 2004; 47 (1): 65-75.

Joborn A, Dorsch M, Olsson C, Westerdahl A, Kjelleberg S. Carnobacterium inhibens sp. nov. Isolated from the Intestine of Atlantic Salmon (Salmo solar). International Journal of Systematic Bacteriology 1999; 49: 1891-1898.

Chen M, Ma LQ, Harris WG. Baseline Concentration of 15 Trace Elements in Florida Surface Soils. Journal of Environmental Quality 1999; 28 (4): 1173-1181.




DOI: http://dx.doi.org/10.11594/jtls.05.03.01

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