Isolation and Characterization Compounds From Hexane and Ethyl Acetate Fractions of Peperomia pellucida L.

Authors

  • Sri Hartati Research Centre for Chemistry
  • Marissa Angelina Research Centre for Chemistry LIPI
  • Indah Dewiyanti Research centre for chemistry LIPI
  • Lia Meilawati research centre for chemistry LIPI

DOI:

https://doi.org/10.11594/jtls.05.03.02

Abstract

      Peperomia pellucida was used traditionally in Indonesia for health treatment: wounds, boils, pimples, abscesses, abdominal pain, colic, gout, kidney, rheumatic pain, fatigue headache, furuncles, conjunctivitis and anti dermatogenic and also for dengue treatment. The isolation compounds from hexane and ethyl acetate fractions of Peperomia pellucida L. are  conducted by maceration of the dry herbs sample with methanol and partition with hexane, ethyl acetate, butanol and water.The hexane and ethyl acetate fractions were fractionated by gravitation column chromatography and eluted successively with hexane, ethyl acetate and methanol by the gradient. The structure was elucidated base on spectroscopy data of NMR proton and carbon for one and two dimension, LC-MS and FT-IR. The isolation founded three compounds are stigmasterol, analogue of pheophytin and b-sitosterol-D-glucopyranoside.

References

Bojo, A. C., Albana-Gracia, E., Poesidio G.N.,1994, The antibacterial activity of Peperomia pellucida (L) HBK( Piperaceae) , Asia life Sciences, 3, 35-44.

Joaquim de C. Bayma , Mara Silvia P. Arruda, Adolfo H. MuÈ ller, Alberto C. Arruda, Williams C. Canto, 2000, A dimeric ArC2 compound from Peperomia pellucida, Phytochemistry, 55, 779 -782.

Khan M.R., A.D. Omoloso, 2002, Antibacterial activity of Hygrophila stricta and Peperomia pellucida, Fitoterapia 73. 251-254.

Arrigoni-Blank M. d. F., Oliveira R. L. B., Mendes S. S., Silva P. d. A., Antoniolli Â. R., Vilar J. C., Cavalcanti S. C. d. H. and Blank A. F., 2002, Seed germination, phenology, and antiedematogenic activity of Peperomia pellucida (L.) H. B. K. BMC Pharmacology, 2, 1-8. (http//www.biomed.central.com/1471-2210/2/12)

Azyba Peter I., A. Adedeji, M. Ekor, O. Adeyemi, 2001, Analgesic activity of Peperomia pellucida aerial parts in mice, Fitoterapia, 72,. 57-58.

Arrigoni-Blank M. de F., Dmitrieva E. G., Franzotti E. M., Antoniolli A. R., Andrade M. R., Marchioro M., 2004, Anti-inflammatory and analgesic activity of Peperomia pellucida (L.) HBK (Piperaceae), Journal of Ethnopharmacology, 91, 215–218.

Der-Jiun Ooi, Shahid Iqbal and Maznah Ismail, 2012, Proximate Composition, Nutritional Attributes and Mineral Composition of Peperomia pellucida L. (Ketumpangan Air), Grown in Malaysia, Molecules, 17, 11139-11145.

Wei L. S. , Wee W. , Siong J. Y. F. , and Syamsumir.D. F., 2011, Characterization of Anticancer, Antimicrobial, Antioxidant Properties and Chemical Compositions of Peperomia pellucida Leaf Extract, Acta Medica Iranica, Vol. 49, No. 10, 670-674.

Xu Su, Na Li,, Meng-Meng Ning, Cai-Hong Zhou, Qiao-Rong Yang, and Ming-Wei Wang, 2006, Bioactive Compounds from Peperomia pellucida, American Chemical Society and American Society of Pharmacognosy Published on Web 02/07/2006.

Bayma J. de C., Arruda M.S. P., Müller A. H., Arruda A.C., Canto W. C., 2000, A Dimeric ARC2 Compound from Peperomia pellucida. Phytochemistry, 55, 779-782.

de Lira PN, da Silva JK, Andrade EH, Sousa PJ, Silva NN, Maia JG., 2009, Essential oil composition of three Peperomia species from the Amazon, Brazil. Nat Prod Commun. Mar;4(3):427-30.

Rojas –Martinez R., Arrieta J., Cruz-Antonio, Arrieta-Baez D., Velazquez-Mendes A. M. and Sanchez-Mendoza M. E., 2013, Dillapiole, Isolated from Peperomia pellucida, Show Gastroprotector Activity Agiants Ethanol-Induced Gastric Lesion in Wistar Rats, Molecules18,11327-11337; doi:10.3390/molecules 180911327.

Ngueguim f. T., Khan M. P., DonfackJ. H., Tewari D., Dimo T., Kamtchouing, P., Maurya R., Chamttopadhyay, 2013, Ethanol Extract of Peperomia pellucida (Piperaceae) Promote Fracture Healing by an Anabolic Effect on Osteoblasts, Journal of Ethnopharmacology,148, 62-68.

Li H., Lina Li, Zheng Q.,Kuroda C., and Wang Q., 2012, Phaeophytin Analogues from Ligularia knorringiana, Molecules, 17, 5219-5224; doi; 10.3390/moleculles17055219.

Lee TH., Lu C K., Kuo YH., Lo J M., and Lee CK., 2008, Unexpected Novel Pheophytin Peroxides from the Leaves of Biden pilosa, Helvetica Chimica Acta, Vol.91, 79 – 84.

Bai, H.,Li, S., Yin , F. And Hu , L., 2005, J. Nat. Prod. 60, ,1159-1163.

Silverstein, R.M., Bassler, G.C., and Morrill, T.C.,1991, Spectrometric Identification of Organic Compounds, Singapore, 221.

Goal, L. J. And Akihisa, T.,(1997), Analysis of Sterols, Blackie Academic & Professional, London and New York.

Downloads

Published

2015-09-07

Issue

Section

Articles